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Protocol: Rye B Agar (For sporulation) for 1 liter

Follow steps 1 and 2 from above.

  1. Boil rye grain (do not blenderize) for one hour in enough additional distilled water to cover the grain (about 1 inch above). Do this in a 2 liter beaker with foil to cover top. Check water level regularly, add more water if needed.
  2. Filter through 4 thicknesses of cheese cloth, squeezing gently to remove residual liquid. Discard cheese cloth and grain.
  3. Combine the supernatants.
  4. Add 15g of Difco Bacto Agar, 20g of sucrose and 0.05g of b-sitosterol. Adjust volume to 1L.
  5. Autoclave at 15 psi for 20 min.

Reference: Caten, C. E. and J. L. Jinks. 1968. Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Can. J. Bot. 46: 329-348.

infected tubers

infected tomatoes