Protocol: Rye B Agar (For sporulation) for 1 liter
Follow steps 1 and 2 from above.
- Boil rye grain (do not blenderize) for one hour in enough additional distilled water to cover the grain (about 1 inch above). Do this in a 2 liter beaker with foil to cover top. Check water level regularly, add more water if needed.
- Filter through 4 thicknesses of cheese cloth, squeezing gently to remove residual liquid. Discard cheese cloth and grain.
- Combine the supernatants.
- Add 15g of Difco Bacto Agar, 20g of sucrose and 0.05g of b-sitosterol. Adjust volume to 1L.
- Autoclave at 15 psi for 20 min.
Reference: Caten, C. E. and J. L. Jinks. 1968. Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Can. J. Bot. 46: 329-348.
