Protocol: CULTURE RETRIEVAL FROM –135 oC
Need: Big blues freezer gloves, styrofoam container with ice, tweezers, sterile filter paper.
Immediately after removing tubes from –135C, place them on ice
Wait 30-60 minutes then remove the plugs from the tubes.
Put the plugs on sterile filter paper to remove excess Glycerol.
Distribute the plugs on two plates of Rye B.
Label the plates with the isolate number and date.
Leave the plates at 18C.
