Welcome to the Lazarowitz lab!
Our overall goal is to identify the molecular and cellular events, which underlie the ability of viruses to infect and spread within a plant to cause disease. Because viruses are obligate intracellular parasites, defining these events can not only lead to the rational development of anti-viral strategies, it also provides insights into how the host regulates gene expression, signal transduction and macromolecular trafficking. For plant viruses, an essential element of their disease potential is their ability to cross the barrier of the plant cell wall to invade the host. Our research has focused on a novel class of proteins encoded by plant viruses: ‘movement proteins’, which play a key role in coordinating replication of the virus genome with its directed transport to and across the plant cell wall. We use the model plant Arabidopsis thaliana and two model plant viruses: the DNA geminivirus Cabbage leaf curl virus (CaLCuV), and the RNA tobamovirus Tobacco mosaic virus (TMV). Employing a variety of approaches –– from molecular and classical genetics to biochemistry, structural biology, confocal imaging, robust transient expression assays and functional genomics –– we have established the current model for how geminivirus movement proteins act to transport the viral genome within and between cells.
Our research findings have lead us identify two essential macromolecular transport pathways, which are pirated by plant viruses to transport viral genomes, and to now focus on investigating the role of these trafficking pathways in plant growth and development, as well as in virus movement:
The regulation of nuclear import and export in plant cells.
The role of vesicle trafficking in plant cell-cell communication.