Skip to main content
more options

Protocol: 10% Unclarified V8 Agar

  1. Combine 100 ml V8 juice and 900 ml of distilled water.
  2. Add 1g CaCO3 and 0.05 g b-sitosterol and mix well. Leave stir bar in the flask for later mixing.
  3. Add 15g of agar.
  4. Autoclave at 15 psi for 20 minutes.
  5. Stir medium while dispensing to insure good mixing of CaCO3.

20% : 200 ml V8, 800ml d. water, 2g CaCO3, and 0.05g b-sitosterol.

15% : 150 ml V8, 850 ml d. water, 1.5g CaCO3, and 0.05g b-sitosterol.

Reference: Miller, P. M. 1955. V-8 juice agar as a general purpose medium for fungi and bacteria. Phytopathology 45:461-462.

infected tubers

infected tomatoes