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Protocol: Slants for Mineral Oil Storage

Slants are Rye A agar (see how to make them at the end of the Rye A agar directions).

Place small actively growing plug of mycelium at the bottom of the slant ( the plug has to be able to fit through the mouth of the test tube ).

When the mycelium has grown to cover almost all the slant, cover it with mineral oil ( the mineral oil must be autoclaved twice within 24 hours for 30 min. each time ). The agar and mycelium should be covered so fill up the tube with oil to at least 1.5 cm above the agar.

Note: Make sure you use autoclaved pipettes. 

infected tubers

infected tomatoes