Protocol: Rye A SLANTS
- Follow steps 1-6 of Rye A protocol.
- Heat agar on hot plate until agar melts.
- Dispense desired volume into tubes.
- Cap the tubes and autoclave them 15 psi for 15 min.
- Lay the racks on their sides on a slant maker.
*For Rye A + antibacterials add the antibacterial solution just prior to pouring the plates ( do not add when agar is too hot - about 50 C is okay).

