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Protocol: Rye A SLANTS

  1. Follow steps 1-6 of Rye A protocol.
  2. Heat agar on hot plate until agar melts.
  3. Dispense desired volume into tubes.
  4. Cap the tubes and autoclave them 15 psi for 15 min.
  5. Lay the racks on their sides on a slant maker.

*For Rye A + antibacterials add the antibacterial solution just prior to pouring the plates ( do not add when agar is too hot - about 50 C is okay).

infected tubers

infected tomatoes