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Protocol: Mating type determination

A small agar plug with mycelium of the sample is placed in the center of each of two rye A or rye B plates. On one plate, an agar plug with mycelium of a known A1 strain (usually US940501) is placed on either side of the sample. On the other plate, the same thing is done, this time using a known A2 strain (usually US 940480). Within 5-10 days, observe for the presence of oospores. If oospores are seen on the plate with the known A1 but not on the plate with the known A2, the sample is A2. If the opposite is seen, the sample is A1.


infected tubers

infected tomatoes