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Protocol: 10% Clarified V8 Agar 

  1. Mix 1.5g CaCO3 with 150 ml V8 juice.
  2. Stir to dissolve the CaCO3 .
  3. Clarify V8 juice by spinning 2 full centrifuge tubes of V8 on the benchtop centrifuge at full speed for 5 minutes.
  4. Combine 100 ml of the clarified supernatant and 900 ml of water.
  5. Add 0.05 g of b-sitosterol , 15 g of agar and mix briefly.
  6. Autoclave at 15 psi for 20 minutes.

Note: This media is good for determining mating types.

infected tubers

infected tomatoes